The existence of cancer stem cells in hepatocellular carcinoma (also known as HCC), which is a cancer of the liver, has been confirmed. This discovery was made possible by characterizing certain cells based on the information gathered from the Hoechst 33342 dye used to stain stem cells. This stain is easy to use on stem cells since it is easily “excited” by ultraviolet light. Recent discoveries in microRNA biology have revealed that these types of RNA's do indeed play an important role in the development of embryos and the formation of tumors. This type of RNA protein is“noncoding”and made up of 19 to 25 nucleotides in length. They regulate gene expression by inhibiting and transforming the layers of mRNAs through base-pairing. However, it is still unclear which microRNAs actually take part in the conversion of normal cells into tumor cells during the production of cancer in the liver.
Cancer stem cells have been identified in cells located in the blood and in solid tumors, including hepatocellular carcinoma (HCC). The isolation and characterization of cancer stem cells are usually based on the presence of stem cell markers. Although, in many tissues, certain markers of bodily stem cells are still unclear. There have been many attempts made to identify cancer stem cells in tumors, based on the results from the use of the Hoechst 33342 dye. The ability to isolate these cells by sorting them, makes it possible to improve both normal bodily stem cells and cancer stem cells without the use of stem cell markers.
Researchers used a gene knockout mouse model to show that microRNAs may be critical regulators of the development of organs in embryonic stem cells. However, data suggests that “dysregulation” of these microRNAs occurs in a many types of cancers, such as lung, colon, and liver. The effects of these specific RNAs in both cancer production and the differences of normal stem cells suggest that microRNA may be involved in the transformation of normal stem cells into cancer stem cells.
Hepatocellular carcinoma is one of the most malignant tumors in existence. However, when researchers divide certain population cells, the presence of liver cancer stem cells in many hepatocellular carcinomas cell lines can be verified using this technique. However, few experiments have focused on the characterization of population cells isolated from HCC cells. The researchers in this study saw that if normal stem cells and liver cancer stem cells could be enriched when the side population cells were isolated, then they could create an in vitro model to determine whether this type of liver cancer could develop through these types of bodily cells.
No comments:
Post a Comment